Review



miltenyi il 10 secretion assay  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Miltenyi Biotec miltenyi il 10 secretion assay
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Miltenyi Il 10 Secretion Assay, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miltenyi il 10 secretion assay/product/Miltenyi Biotec
    Average 97 stars, based on 5 article reviews
    miltenyi il 10 secretion assay - by Bioz Stars, 2026-06
    97/100 stars

    Images

    1) Product Images from "Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging"

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    Journal: bioRxiv

    doi: 10.64898/2026.05.05.723043

    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Figure Legend Snippet: A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Techniques Used: Staining, Expressing, Activation Assay, Quantitative Proteomics, Immunofluorescence

    A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Figure Legend Snippet: A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Techniques Used: Expressing, Gene Expression, Activity Assay, Comparison



    Similar Products

    miltenyi il 10 secretion assay  (Miltenyi Biotec)
    97
    Miltenyi Biotec miltenyi il 10 secretion assay
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Miltenyi Il 10 Secretion Assay, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miltenyi il 10 secretion assay/product/Miltenyi Biotec
    Average 97 stars, based on 1 article reviews
    miltenyi il 10 secretion assay - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier
    pe annexin v apoptosis detection kit  (Vazyme Biotech Co)
    96
    Vazyme Biotech Co pe annexin v apoptosis detection kit
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Pe Annexin V Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe annexin v apoptosis detection kit/product/Vazyme Biotech Co
    Average 96 stars, based on 1 article reviews
    pe annexin v apoptosis detection kit - by Bioz Stars, 2026-06
    96/100 stars
    		  Buy from Supplier
    annexin v fitc pi apoptosis detection kit  (Beyotime)
    97
    Beyotime annexin v fitc pi apoptosis detection kit
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Beyotime
    Average 97 stars, based on 1 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier
    annexin v fitc apoptosis detection kit  (Beyotime)
    97
    Beyotime annexin v fitc apoptosis detection kit
    Knockdown of USP52 causes reduced viability and proliferation, cell cycle arrest, increased <t>apoptosis,</t> and impaired migration and invasion of ccRCC cells. (A) MTT assay data indicates that USP52 deficiency results in reduced viability of 786-O and Caki-1 cells. (B and C) EdU assay results suggest that USP52-deficient 786-O and Caki-1 cells are less proliferative than their control counterparts. (D and E) 7-AAD staining data show that USP52 deficiency causes cell cycle arrest in 786-O and Caki-1 cells. (F, G) <t>Annexin</t> V/PI staining results suggest that USP52 knockdown leads to extra apoptosis in 786-O and Caki-1 cells. (H and I) Transwell assay results show that the migration of 786-O and Caki-1 cells is attenuated by USP52 depletion. (J and K) Transwell assay results indicate that the invasion of USP52-deficient 786-O and Caki-1 cells is mitigated compared to control cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.
    Annexin V Fitc Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc apoptosis detection kit/product/Beyotime
    Average 97 stars, based on 1 article reviews
    annexin v fitc apoptosis detection kit - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier
    annexin v fitc cell apoptosis detection kit  (Beyotime)
    97
    Beyotime annexin v fitc cell apoptosis detection kit
    Knockdown of USP52 causes reduced viability and proliferation, cell cycle arrest, increased <t>apoptosis,</t> and impaired migration and invasion of ccRCC cells. (A) MTT assay data indicates that USP52 deficiency results in reduced viability of 786-O and Caki-1 cells. (B and C) EdU assay results suggest that USP52-deficient 786-O and Caki-1 cells are less proliferative than their control counterparts. (D and E) 7-AAD staining data show that USP52 deficiency causes cell cycle arrest in 786-O and Caki-1 cells. (F, G) <t>Annexin</t> V/PI staining results suggest that USP52 knockdown leads to extra apoptosis in 786-O and Caki-1 cells. (H and I) Transwell assay results show that the migration of 786-O and Caki-1 cells is attenuated by USP52 depletion. (J and K) Transwell assay results indicate that the invasion of USP52-deficient 786-O and Caki-1 cells is mitigated compared to control cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.
    Annexin V Fitc Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc cell apoptosis detection kit/product/Beyotime
    Average 97 stars, based on 1 article reviews
    annexin v fitc cell apoptosis detection kit - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Journal: bioRxiv

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    doi: 10.64898/2026.05.05.723043

    Figure Lengend Snippet: A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Article Snippet: On day 13, IL-10 secretion was assessed using the Miltenyi IL-10 Secretion Assay (Cat# 130-090-434).

    Techniques: Staining, Expressing, Activation Assay, Quantitative Proteomics, Immunofluorescence

    A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Journal: bioRxiv

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    doi: 10.64898/2026.05.05.723043

    Figure Lengend Snippet: A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Article Snippet: On day 13, IL-10 secretion was assessed using the Miltenyi IL-10 Secretion Assay (Cat# 130-090-434).

    Techniques: Expressing, Gene Expression, Activity Assay, Comparison

    Knockdown of USP52 causes reduced viability and proliferation, cell cycle arrest, increased apoptosis, and impaired migration and invasion of ccRCC cells. (A) MTT assay data indicates that USP52 deficiency results in reduced viability of 786-O and Caki-1 cells. (B and C) EdU assay results suggest that USP52-deficient 786-O and Caki-1 cells are less proliferative than their control counterparts. (D and E) 7-AAD staining data show that USP52 deficiency causes cell cycle arrest in 786-O and Caki-1 cells. (F, G) Annexin V/PI staining results suggest that USP52 knockdown leads to extra apoptosis in 786-O and Caki-1 cells. (H and I) Transwell assay results show that the migration of 786-O and Caki-1 cells is attenuated by USP52 depletion. (J and K) Transwell assay results indicate that the invasion of USP52-deficient 786-O and Caki-1 cells is mitigated compared to control cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.

    Journal: Translational Oncology

    Article Title: USP52 promotes clear cell renal carcinoma progression by deubiquitinating and stabilizing CORO6

    doi: 10.1016/j.tranon.2026.102773

    Figure Lengend Snippet: Knockdown of USP52 causes reduced viability and proliferation, cell cycle arrest, increased apoptosis, and impaired migration and invasion of ccRCC cells. (A) MTT assay data indicates that USP52 deficiency results in reduced viability of 786-O and Caki-1 cells. (B and C) EdU assay results suggest that USP52-deficient 786-O and Caki-1 cells are less proliferative than their control counterparts. (D and E) 7-AAD staining data show that USP52 deficiency causes cell cycle arrest in 786-O and Caki-1 cells. (F, G) Annexin V/PI staining results suggest that USP52 knockdown leads to extra apoptosis in 786-O and Caki-1 cells. (H and I) Transwell assay results show that the migration of 786-O and Caki-1 cells is attenuated by USP52 depletion. (J and K) Transwell assay results indicate that the invasion of USP52-deficient 786-O and Caki-1 cells is mitigated compared to control cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.

    Article Snippet: Cell apoptosis was assessed using the Annexin V-FITC apoptosis detection kit (Beyotime Biotechnology) following the manufacturer's instructions.

    Techniques: Knockdown, Migration, MTT Assay, EdU Assay, Control, Staining, Transwell Assay

    Overexpression of CORO6 restores the malignancy of USP52-deficient ccRCC cells. (A and B) 7-AAD staining data reveal that excessive CORO6 facilitates cell cycle progression in USP52-deficient 786-O and Caki-1 cells. (C and D) Annexin V/PI staining assay data indicate that excessive CORO6 decreases the apoptosis of 786-O and Caki-1 cells with USP52 deficiency. (E and F) Transwell assay results show that overexpression of CORO6 strengthens the migration of USP52-deficient 786-O and Caki-1 cells. (G and H) Transwell assay results demonstrate that excessive CORO6 enhances the invasion of USP52-deficient 786-O and Caki-1 cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.

    Journal: Translational Oncology

    Article Title: USP52 promotes clear cell renal carcinoma progression by deubiquitinating and stabilizing CORO6

    doi: 10.1016/j.tranon.2026.102773

    Figure Lengend Snippet: Overexpression of CORO6 restores the malignancy of USP52-deficient ccRCC cells. (A and B) 7-AAD staining data reveal that excessive CORO6 facilitates cell cycle progression in USP52-deficient 786-O and Caki-1 cells. (C and D) Annexin V/PI staining assay data indicate that excessive CORO6 decreases the apoptosis of 786-O and Caki-1 cells with USP52 deficiency. (E and F) Transwell assay results show that overexpression of CORO6 strengthens the migration of USP52-deficient 786-O and Caki-1 cells. (G and H) Transwell assay results demonstrate that excessive CORO6 enhances the invasion of USP52-deficient 786-O and Caki-1 cells. One-way ANOVA followed by Tukey's HSD was used for P -value calculation.

    Article Snippet: Cell apoptosis was assessed using the Annexin V-FITC apoptosis detection kit (Beyotime Biotechnology) following the manufacturer's instructions.

    Techniques: Over Expression, Staining, Transwell Assay, Migration